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As described previously (Pinco et al., 2002), green fluorescent protein (GFP)-tagged α4 integrin is functionally normal. GST-tagged proteins containing the disintegrin domains of ADAM12 (wild type and mutant), ADAM19 and ADAM33 were produced exactly as described previously (Tomczuk et al., 2003). Sequences encoding the disintegrin domains of mouse ADAM19 (residues G417-G504) or mouse ADAM 33 (residues P418-G505) in frame with a https://yabancidiziizle.org/ terminal 6Xhistidine tag were generated by PCR.

Distinct ADAMs Selectively Inhibit Integrin-mediated Cell Migration

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(A) Images of cells from the 12-h time point of the experiment shown in Figure 3B. (C) CHO-α4/GFP cells were transfected as indicated and then analyzed in scratch wound assays, as described in the legend to Figure 2, except that 24-well dishes were coated with 10 μg/ml CCBD. (B) CHO-α4/GFP cells were transfected as indicated and then analyzed in scratch wound assays, as described in the legend to Figure 2, except that 24-well dishes were coated with 2 μg/ml CS-1.

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